Time-gated confocal microscopy reveals accumulation of exocyst subunits at the plant-pathogen interface.
Identifieur interne : 000021 ( Main/Exploration ); précédent : 000020; suivant : 000022Time-gated confocal microscopy reveals accumulation of exocyst subunits at the plant-pathogen interface.
Auteurs : Elysa J R. Overdijk [Pays-Bas] ; Han Tang [Pays-Bas] ; Jan Willem Borst [Pays-Bas] ; Francine Govers [Pays-Bas] ; Tijs Ketelaar [Pays-Bas]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2020.
Abstract
Polarized exocytosis is essential for plant development and defence. The exocyst, an octameric protein complex that tethers exocytotic vesicles to the plasma membrane, targets exocytosis. Upon pathogen attack, secreted materials form papillae to halt pathogen penetration. To determine if the exocyst is directly involved in targeting exocytosis to infection sites, information about its localization is instrumental. Here, we investigated exocyst subunit localization in the moss Physcomitrella patens upon pathogen attack and infection by Phytophthora capsici. Time-gated confocal microscopy was used to eliminate autofluorescence of deposited material around infection sites, allowing the visualization of the subcellular localization of exocyst subunits and of v-SNARE Vamp72A1-labelled exocytotic vesicles during infection. This showed that exocyst subunits Sec3a, Sec5b, Sec5d, and Sec6 accumulated at sites of attempted pathogen penetration. Upon pathogen invasion, the exocyst subunits accumulated on the membrane surrounding papilla-like structures and hyphal encasements. Vamp72A1-labelled vesicles were found to localize in the cytoplasm around infection sites. The re-localization of exocyst subunits to infection sites suggests that the exocyst is directly involved in facilitating polarized exocytosis during pathogenesis.
DOI: 10.1093/jxb/erz478
PubMed: 31665494
Affiliations:
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Overdijk</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cell Biology, Wageningen University & Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Cell Biology, Wageningen University & Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University & Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University & Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Han" sort="Tang, Han" uniqKey="Tang H" first="Han" last="Tang">Han Tang</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cell Biology, Wageningen University & Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Cell Biology, Wageningen University & Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Borst, Jan Willem" sort="Borst, Jan Willem" uniqKey="Borst J" first="Jan Willem" last="Borst">Jan Willem Borst</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biochemistry, Wageningen University & Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Biochemistry, Wageningen University & Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University & Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University & Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Ketelaar, Tijs" sort="Ketelaar, Tijs" uniqKey="Ketelaar T" first="Tijs" last="Ketelaar">Tijs Ketelaar</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cell Biology, Wageningen University & Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Cell Biology, Wageningen University & Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polarized exocytosis is essential for plant development and defence. The exocyst, an octameric protein complex that tethers exocytotic vesicles to the plasma membrane, targets exocytosis. Upon pathogen attack, secreted materials form papillae to halt pathogen penetration. To determine if the exocyst is directly involved in targeting exocytosis to infection sites, information about its localization is instrumental. Here, we investigated exocyst subunit localization in the moss Physcomitrella patens upon pathogen attack and infection by Phytophthora capsici. Time-gated confocal microscopy was used to eliminate autofluorescence of deposited material around infection sites, allowing the visualization of the subcellular localization of exocyst subunits and of v-SNARE Vamp72A1-labelled exocytotic vesicles during infection. This showed that exocyst subunits Sec3a, Sec5b, Sec5d, and Sec6 accumulated at sites of attempted pathogen penetration. Upon pathogen invasion, the exocyst subunits accumulated on the membrane surrounding papilla-like structures and hyphal encasements. Vamp72A1-labelled vesicles were found to localize in the cytoplasm around infection sites. The re-localization of exocyst subunits to infection sites suggests that the exocyst is directly involved in facilitating polarized exocytosis during pathogenesis.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31665494</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>71</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>01</Month><Day>23</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Time-gated confocal microscopy reveals accumulation of exocyst subunits at the plant-pathogen interface.</ArticleTitle><Pagination><MedlinePgn>837-849</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erz478</ELocationID><Abstract><AbstractText>Polarized exocytosis is essential for plant development and defence. The exocyst, an octameric protein complex that tethers exocytotic vesicles to the plasma membrane, targets exocytosis. Upon pathogen attack, secreted materials form papillae to halt pathogen penetration. To determine if the exocyst is directly involved in targeting exocytosis to infection sites, information about its localization is instrumental. Here, we investigated exocyst subunit localization in the moss Physcomitrella patens upon pathogen attack and infection by Phytophthora capsici. Time-gated confocal microscopy was used to eliminate autofluorescence of deposited material around infection sites, allowing the visualization of the subcellular localization of exocyst subunits and of v-SNARE Vamp72A1-labelled exocytotic vesicles during infection. This showed that exocyst subunits Sec3a, Sec5b, Sec5d, and Sec6 accumulated at sites of attempted pathogen penetration. Upon pathogen invasion, the exocyst subunits accumulated on the membrane surrounding papilla-like structures and hyphal encasements. Vamp72A1-labelled vesicles were found to localize in the cytoplasm around infection sites. The re-localization of exocyst subunits to infection sites suggests that the exocyst is directly involved in facilitating polarized exocytosis during pathogenesis.</AbstractText><CopyrightInformation>© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Overdijk</LastName><ForeName>Elysa J R</ForeName><Initials>EJR</Initials><AffiliationInfo><Affiliation>Laboratory of Cell Biology, Wageningen University & Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University & Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Han</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Cell Biology, Wageningen University & Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borst</LastName><ForeName>Jan Willem</ForeName><Initials>JW</Initials><AffiliationInfo><Affiliation>Laboratory of Biochemistry, Wageningen University & Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Govers</LastName><ForeName>Francine</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University & Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ketelaar</LastName><ForeName>Tijs</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Cell Biology, Wageningen University & Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Physcomitrella patens
</Keyword><Keyword MajorTopicYN="Y">Phytophthora capsici
</Keyword><Keyword MajorTopicYN="Y"> Exocyst</Keyword><Keyword MajorTopicYN="Y">SNARE</Keyword><Keyword MajorTopicYN="Y">Vamp72</Keyword><Keyword MajorTopicYN="Y">exocytosis</Keyword><Keyword MajorTopicYN="Y">plant defence</Keyword><Keyword MajorTopicYN="Y">time-gated confocal microscopy</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>10</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>10</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31665494</ArticleId><ArticleId IdType="pii">5607674</ArticleId><ArticleId IdType="doi">10.1093/jxb/erz478</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Overdijk, Elysa J R" sort="Overdijk, Elysa J R" uniqKey="Overdijk E" first="Elysa J R" last="Overdijk">Elysa J R. Overdijk</name></noRegion><name sortKey="Borst, Jan Willem" sort="Borst, Jan Willem" uniqKey="Borst J" first="Jan Willem" last="Borst">Jan Willem Borst</name><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><name sortKey="Ketelaar, Tijs" sort="Ketelaar, Tijs" uniqKey="Ketelaar T" first="Tijs" last="Ketelaar">Tijs Ketelaar</name><name sortKey="Overdijk, Elysa J R" sort="Overdijk, Elysa J R" uniqKey="Overdijk E" first="Elysa J R" last="Overdijk">Elysa J R. Overdijk</name><name sortKey="Tang, Han" sort="Tang, Han" uniqKey="Tang H" first="Han" last="Tang">Han Tang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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